Submitted by aly01 on Sun, 2007-01-21 21:56 Hi, I am having problems understanding how to make very dilute solutions like 1:1000, 1:2000 and 1:5000 . Can someone please explain this to me. :-\ Thanks, Re: Dilutions Permalink Submitted by Chemistry Tutor on Tue, 2007-01-23 14:11 Well are you doing it in the lab or do you need to do it for a calculation? At the very simplest level, in a lab to do a 1/10 dilution you could take 1 milliliter of the original sample and add 9 milliliters of solvent (most likely water) this would be a 1: 10 dilution you would most likely use a micropipette to do a dilution at the levels you are needing. it also depends on the accuracy you need. the proper technique for a 1:1000 dilution would require you to take 0.01 mL of sample and dilute it in a 10mL volumetric flask. the general equation is M1V1 = M2V2 Log in or register to post comments
Re: Dilutions
Well are you doing it in the lab or do you need to do it for a calculation?
At the very simplest level, in a lab to do a 1/10 dilution
you could take 1 milliliter of the original sample and add 9 milliliters of solvent (most likely water)
this would be a 1: 10 dilution
you would most likely use a micropipette to do a dilution at the levels you are needing. it also depends on the accuracy you need.
the proper technique for a 1:1000 dilution would require you
to take 0.01 mL of sample and dilute it in a 10mL volumetric flask.
the general equation is
M1V1 = M2V2