How do I do a serial dilution calculation?


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I am having problems understanding how to make  very dilute solutions like 1:1000, 1:2000 and 1:5000 .
Can someone please explain this to me. :-\


Well are you doing it in the lab or do you need to do it for a calculation?

At the very simplest level, in a lab to do a 1/10 dilution

you could take 1 milliliter of the original sample and add 9 milliliters of solvent (most likely water)

this would be a 1: 10 dilution

you would most likely use a micropipette to do a dilution at the levels you are needing. it also depends on the accuracy you need.

the proper technique for a 1:1000 dilution would require you

to take 0.01 mL of sample and dilute it in a 10mL volumetric flask.

the general equation is

M1V1 = M2V2